Substrate recognition and induced DNA deformation by transposase at the target-capture stage of Tn10 transposition.

@article{Pribil2000SubstrateRA,
  title={Substrate recognition and induced DNA deformation by transposase at the target-capture stage of Tn10 transposition.},
  author={Patrick A Pribil and David B. Haniford},
  journal={Journal of molecular biology},
  year={2000},
  volume={303 2},
  pages={145-59}
}
The bacterial transposon Tn10 inserts preferentially into sites that conform to a 9 bp consensus sequence: 5' NGCTNAGCN 3'. However, this sequence is not on its own sufficient to confer target specificity as the base-pairs flanking this sequence also contribute significantly to target-site selection. We have performed a series of "contact-probing experiments" to define directly the protein-DNA interactions that govern target-site selection in the Tn10 system. The HisG1 hotspot for Tn10… CONTINUE READING
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