Substrate preparation for reliable imaging of DNA molecules with the scanning force microscope.

@article{Vesenka1992SubstratePF,
  title={Substrate preparation for reliable imaging of DNA molecules with the scanning force microscope.},
  author={J P Vesenka and Martin Guthold and Chang Long Tang and Dami{\'a}n Keller and E Delaine and C. Bustamante},
  journal={Ultramicroscopy},
  year={1992},
  volume={42-44 ( Pt B)},
  pages={1243-9}
}
A simple method of substrate preparation for imaging circular DNA molecules with the scanning force microscope (SFM) is presented. These biomolecules are adsorbed onto mica that has been soaked in magnesium acetate, sonicated and glow-discharged. The stylus-sample forces that may be endured before sample damage occurs depends on the ambient relative humidity. Images of circular DNA molecules have been obtained routinely using tips specially modified by an electron beam with a radius of… CONTINUE READING

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