Substrate-inducible microsomal aryl hydroxylase in mammalian cell culture. I. Assay and properties of induced enzyme.

@article{Nebert1968SubstrateinducibleMA,
  title={Substrate-inducible microsomal aryl hydroxylase in mammalian cell culture. I. Assay and properties of induced enzyme.},
  author={Daniel W. Nebert and H. V. Gelboin},
  journal={The Journal of biological chemistry},
  year={1968},
  volume={243 23},
  pages={6242-9}
}
The aryl hydroxylase enzyme system is inducible in hamster fetus cell cultures. The enzyme system is localized in the 105,000 X g pellet (“microsomal fraction”), has an absolute requirement for NADPH and molecular 02, a pH optimum of pH 7.5, and a partial requirement for divalent cations. Exposure to carbon monoxide reduces the enzyme activity. Treatment with ethylenediaminetetraacetate or trypsin completely prevents enzymatic activity. The K,,, for the hydroxylase is approximately 0.6 PM with… CONTINUE READING

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adult organisms

  • P. SIMS
  • Biochem. Pharmacol.,
  • 1967
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