Substrate evokes translocation of both domains in the mitochondrial processing peptidase alpha-subunit during which the C-terminus acts as a stabilizing element.

Abstract

All three tryptophan residues in alpha-subunit of mitochondrial processing peptidase (MPP) were subsequently substituted. While substitutions of Trp223 led to misfolded non-functional protein, mutations of Trp147 and/or Trp481 did not affect the enzyme processing activity. Thus, fluorescence properties of the mutants with fewer tryptophans were used for… (More)

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Cite this paper

@article{Janata2004SubstrateET, title={Substrate evokes translocation of both domains in the mitochondrial processing peptidase alpha-subunit during which the C-terminus acts as a stabilizing element.}, author={Jiri Janata and Kl{\'a}ra Hol{\'a} and Martin Kubala and Oleksandr Gakh and Natalya Parkhomenko and Anna Matu{\vs}kov{\'a} and Eva Kutějov{\'a} and Ev{\vz}en Amler}, journal={Biochemical and biophysical research communications}, year={2004}, volume={316 1}, pages={211-7} }