Substrate-dependent regulation of intracellular amino acid concentrations in cultured bovine aortic endothelial cells.

@article{Baydoun1990SubstratedependentRO,
  title={Substrate-dependent regulation of intracellular amino acid concentrations in cultured bovine aortic endothelial cells.},
  author={Anwar R. Baydoun and Peter W. Emery and Jeremy D. Pearson and Giovanni E. Mann},
  journal={Biochemical and biophysical research communications},
  year={1990},
  volume={173 3},
  pages={940-8}
}
Amino acid deprivation induces adaptive changes in amino acid transport and the intracellular amino acid pool in cultured cells. In this study intracellular amino acid levels were determined in cultured bovine aortic endothelial cells (EC) deprived of L-arginine or total amino acids for 1, 3, 6 and 24 h. Amino acid concentrations were analyzed by reverse phase HPLC after precolumn derivatisation. Under normal culture conditions levels of L-arginine L-citrulline, total essential and non… CONTINUE READING