Substrate-dependent effect of acetonitrile on human liver microsomal cytochrome P450 2C9 (CYP2C9) activity.

@article{Tang2000SubstratedependentEO,
  title={Substrate-dependent effect of acetonitrile on human liver microsomal cytochrome P450 2C9 (CYP2C9) activity.},
  author={Cuyue Tang and Magang Shou and A. David Rodrigues},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2000},
  volume={28 5},
  pages={567-72}
}
Acetonitrile is an organic solvent commonly used to increase the solubility of lipophilic substrates for in vitro studies. In this study, we examined its effect on four reactions (diclofenac hydroxylation, tolbutamide methyl hydroxylation, phenytoin hydroxylation, and celecoxib methyl hydroxylation) catalyzed by human liver microsomes and by the recombinant CYP2C9. In both cases, the effect of acetonitrile on activity was found to be substrate-dependent. Namely, it increased diclofenac 4… CONTINUE READING

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