Substrate-bound Structures of Benzylsuccinate Synthase Reveal How Toluene Is Activated in Anaerobic Hydrocarbon Degradation*

@article{Funk2015SubstrateboundSO,
  title={Substrate-bound Structures of Benzylsuccinate Synthase Reveal How Toluene Is Activated in Anaerobic Hydrocarbon Degradation*},
  author={M. Funk and E. Marsh and C. Drennan},
  journal={The Journal of Biological Chemistry},
  year={2015},
  volume={290},
  pages={22398 - 22408}
}
Background: Benzylsuccinate synthase (BSS) catalyzes the formation of a C—C bond between toluene and fumarate by a radical mechanism. Results: BSS binds substrates in a buried active site and uses conformational changes to gate access. Conclusion: BSS and related enzymes precisely position substrates for chemistry. Significance: These structures reveal the molecular basis for BSS radical chemistry and substrate specificity. Various bacteria perform anaerobic degradation of small hydrocarbons as… Expand
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ABSTRACT The pathway of anaerobic toluene oxidation to benzoyl coenzyme A (benzoyl-CoA) consists of an initial reaction catalyzed by benzylsuccinate synthase, a glycyl radical enzyme adding theExpand
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