Substrate and phenobarbital inducible aflatoxin-4-hydroxylation and aflatoxin metabolism by rat liver microsomes.

  title={Substrate and phenobarbital inducible aflatoxin-4-hydroxylation and aflatoxin metabolism by rat liver microsomes.},
  author={Johannes C. Schabort and M. Steyn},
  journal={Biochemical pharmacology},
  volume={18 9},
Comparative oxidative metabolism of aflatoxin B1 and palmotoxins B0 and G0 by rat liver microsomal fractions.
  • O. BassirB. Emerole
  • Chemistry, Biology
    Xenobiotica; the fate of foreign compounds in biological systems
  • 1973
Aflatoxin B1 showed a higher rate of metabolism than the two other substances, and the two derivatives of palmotoxin B0 have not been characterized, as they are more polar than the parent compound.
On the binding of aflatoxin B 1 and its metabolites to hepatic microsomes.
  • H. Gurtoo
  • Biology, Chemistry
    Biochemical and biophysical research communications
  • 1973
Temporal patterns of covalent DNA adducts in rat liver after single and multiple doses of aflatoxin B1.
Administration of multiple doses of aflatoxin B1, using a regimen shown to produce a high incidence of hepatocellular carcinoma, caused accumulation of these persistent products in liver DNA over a 14-day period.


Extraction of Aflatoxin from Rat Liver
It has been shown that a single dose of aflatoxin B1 given to a rat will result in acute liver damage and after one month the liver still shows the biliary proliferation and also large hyperchromatic parenchymal cells.
Microbial detoxification of aflatoxin.
Duckling assays showed that detoxification of aflatoxin solutions by B-184 was complete, with no new toxic products being formed.
Objective fluorometric measurement of alfatoxins on TLC plates
Measurement of the solid state fluorescence of aflatoxins on silica gel-coated TLC plates on a densitometer equipped for fluorescence measurements showed a linear relationship between peak areas and
Assay of plant tyrosinase by agar-gel double diffusion and electro-osmophoresis.
Agar-gel double diffusion and electro-osmophoresis are adapted as rapid and sensitive assay methods for tyrosinase, a commonly occurring phenolase that can oxidize a variety of phenols as as tyrosine to the products.