Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex

@article{Liang2007SubstrateRP,
  title={Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex},
  author={Bo Liang and Song Xue and Rebecca M. Terns and Michael P Terns and Hong Li},
  journal={Nature Structural &Molecular Biology},
  year={2007},
  volume={14},
  pages={1189-1195}
}
The most complex RNA pseudouridylases are H/ACA ribonucleoprotein particles, which use a guide RNA for substrate capture and four proteins (Cbf5, Nop10, Gar1 and L7Ae/NHP2) for substrate modification. Here we report the three-dimensional structure of a catalytically deficient archaeal enzyme complex (including the guide RNA and three of the four essential proteins) bound to a substrate RNA. Extensive interactions of Cbf5 with one guide-substrate helix and a guide RNA stem shape the forked guide… CONTINUE READING