Substitution of two residues in the measles virus nucleoprotein results in an impaired self-association.

@article{Karlin2002SubstitutionOT,
  title={Substitution of two residues in the measles virus nucleoprotein results in an impaired self-association.},
  author={David Karlin and Sonia Longhi and Bruno Canard},
  journal={Virology},
  year={2002},
  volume={302 2},
  pages={
          420-32
        }
}
The nucleoprotein (N) of measles virus encapsidates viral genomic RNA to form a helical nucleocapsid. Its strong self-association is a major hurdle in determining its high-resolution structure using X-ray crystallography. We report the bacterial expression, purification, and characterization of a variant N that has lost its ability to form nucleocapsid-like structures after substitution of two residues by polar residues. Using immunoprecipitation, circular dichroism, and limited proteolysis… CONTINUE READING
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