Substantial differences in bias between single-digest and double-digest RAD-seq libraries: A case study.

@article{Flanagan2018SubstantialDI,
  title={Substantial differences in bias between single-digest and double-digest RAD-seq libraries: A case study.},
  author={Sarah P Flanagan and Adam G. Jones},
  journal={Molecular ecology resources},
  year={2018},
  volume={18 2},
  pages={
          264-280
        }
}
The trade-offs of using single-digest vs. double-digest restriction site-associated DNA sequencing (RAD-seq) protocols have been widely discussed. However, no direct empirical comparisons of the two methods have been conducted. Here, we sampled a single population of Gulf pipefish (Syngnathus scovelli) and genotyped 444 individuals using RAD-seq. Sixty individuals were subjected to single-digest RAD-seq (sdRAD-seq), and the remaining 384 individuals were genotyped using a double-digest RAD-seq… CONTINUE READING