Substance P excites GABAergic neurons in the mouse central amygdala through neurokinin 1 receptor activation.

  title={Substance P excites GABAergic neurons in the mouse central amygdala through neurokinin 1 receptor activation.},
  author={Ludmila Sosulina and Christine Strippel and Héctor Romo-Parra and A. Walter and Tatyana G. Kanyshkova and Simone B. Sartori and Maren Denise Lange and Nicolas Singewald and Hans-Christian Pape},
  journal={Journal of neurophysiology},
  volume={114 4},
Substance P (SP) is implicated in stress regulation and affective and anxiety-related behavior. Particularly high expression has been found in the main output region of the amygdala complex, the central amygdala (CE). Here we investigated the cellular mechanisms of SP in CE in vitro, taking advantage of glutamic acid decarboxylase-green fluorescent protein (GAD67-GFP) knockin mice that yield a reliable labeling of GABAergic neurons, which comprise 95% of the neuronal population in the lateral… Expand
Alcohol dependence potentiates substance P/neurokinin-1 receptor signaling in the rat central nucleus of amygdala
Examination of regulation of GABA transmission in the medial subdivision of the central amygdala (CeM) by the SP/NK-1R system, and its neuroadaptation following chronic alcohol exposure confirms that NK-1Rs are promising targets for the treatment of alcohol use disorder. Expand
Substance P: A neuropeptide involved in the psychopathology of anxiety disorders
As SP concentration is found elevated in stressed conditions, several studies have reported that the pharmacological antagonism or genetic depletion of NK-1 receptors results in the anxiolytic response making them a suitable therapeutic target for the treatment of stress and anxiety related disorders. Expand
Substance P effects exclusively on prototypic neurons in mouse globus pallidus
The SP-responsive cells comprise a highly specific cell type in the GP with regard to immunofluorescence, electrophysiology, and projection properties, and could affect the indirect pathway in the way of prototypic neurons, via the action of SP to NK-1R. Expand
Substance P and the Neurokinin-1 Receptor: The New CRF.
The NK1R merits being revisited as a target for the development of novel pharmacotherapeutics for addiction with promising clinical findings for the efficacy of NK1r antagonists on craving in alcoholics, along with recent data suggesting that a number of negative results fromNK1R trials were likely due to insufficient receptor occupancy. Expand
Substance P and neurotensin in the limbic system: Their roles in reinforcement and memory consolidation
A hypothesis is presented that rewarding/reinforcing effects of SP and NT develop by modulating the mesencephalic dopaminergic system, while their mnemonic effects are mediated via the mesENCEphalic dopamine and the basal forebrain cholinergic systems. Expand
A basal ganglia-like cortical–amygdalar–hypothalamic network mediates feeding behavior
The anatomical findings in rats and mice indicate that the INS-PSTN network is organized in a similar manner as the hyperdirect and indirect basal ganglia circuitry. Expand
A balancing act: the role of pro‐ and anti‐stress peptides within the central amygdala in anxiety and alcohol use disorders
The neurocircuitry, neurochemical phenotypes and how pro‐ and anti‐stress peptide systems act within the CeA to regulate anxiety and alcohol seeking are examined, focusing on preclinical observations from animal models. Expand
Gap Junctions Between Striatal D1 Neurons and Cholinergic Interneurons
  • Yuqi Ren, Yang Liu, M. Luo
  • Medicine
  • Frontiers in Cellular Neuroscience
  • 2021
Direct electrical coupling is uncovered between D1-type projection neurons and ChIs, in addition to the chemical transmission between these two major cell types, which contributes to maintaining the tonically active firing patterns of ChIs. Expand
The effects and mechanism of substance P in sedative and hypnotic of isoflurane mice
Objective: To investigate the changes of serum substance P (Substance P, SP) content and its effects on sedation and hypnosis after mice inhale Isoflurane. Methods: 45 mice were randomly divided intoExpand
Psycho-emotional status but not cognition is changed under the combined effect of ionizing radiations at doses related to deep space missions
For the first time, data was received that radiation effects in the doses and composition of interplanetary space (in terrestrial modeling) could be relatively safe for cognitive functions. Expand


Substance P inhibits GABAB receptor signalling in the ventral tegmental area
Investigating the mechanisms underlying SP actions in the VTA shows that in VTA neurons, NK receptor activation closes an inwardly rectifying potassium channel, and moreover inhibits GABAB receptor‐mediated transmission through an interaction that depends upon phospholipase C, intracellular calcium and protein kinase C (PKC). Expand
Anxiety-like behavior is modulated by a discrete subpopulation of interneurons in the basolateral amygdala
The phenotypic and regional selectivity of the lesions was confirmed, and the extent to which the NK(1r) cells of amygdala nuclei contribute to anxiety-like responses was also investigated. Expand
Substance P in the medial amygdala: Emotional stress-sensitive release and modulation of anxiety-related behavior in rats
The MeA is identified as a critical brain area for the involvement of SP transmission in anxiety responses and as a putative site of action for the recently discovered therapeutic effects of SP antagonists in the treatment of stress-related disorders. Expand
Neurokinin-1 Receptor-Expressing Neurons in the Amygdala Modulate Morphine Reward and Anxiety Behaviors in the Mouse
It is suggested that the amygdala is an important area for the effects of SP and the NK1 receptor in the motivational properties of opiates, as well as the control of behaviors related to anxiety. Expand
Substance P stimulates inhibitory synaptic transmission in the guinea pig basolateral amygdala in vitro
A physiological role for SP in the regulation of pathways involved in the control of emotional behaviour is supported, and immunohistochemistry revealed NK1 receptor immunoreactivity to be largely restricted to a subset of GABA interneurones. Expand
Subpopulations of neurokinin 1 receptor-expressing neurons in the rat lateral amygdala display a differential pattern of innervation from distinct glutamatergic afferents
The data add to previous localization studies by describing an unexpected variation between LA and basal nucleus of the amygdala (BA) in the neurochemical phenotype of NK1-expressing neurons and reveal the relative source of glutamatergic inputs that may activate these neurons, which in turn regulate fear and anxiety responses. Expand
Effects of Substance P in the Amygdala, Ventromedial Hypothalamus, and Periaqueductal Gray on Fear-Potentiated Startle
It is suggested that multiple outputs from the amygdala play a critical role in fear-potentiated startle and that SP plays a critical, probably modulatory role, in the MeA to VMH to PAG to the startle pathway based on these and data from others. Expand
Neurosteroids increase tonic GABAergic inhibition in the lateral section of the central amygdala in mice.
Evidence is provided for the existence of tonic GABAergic inhibition generated by the activation of δ-subunit-containing GABA(A)Rs in neurons of the lateral section of the mouse central amygdala (CeAl) and it is shown that neurosteroids play an important role in the modulation of tonics inhibitory signaling in the CeAl. Expand
Postsynaptic actions of substance P on rat periaqueductal grey neurons in vitro
Results indicate that substance P acts via NK1, NK2 and NK3 receptors to excite subpopulations of opioid-sensitive and insensitive PAG neurons by increasing a non-selective cation conductance and by reducing a K- current, and has anti-opioid actions that are largely mediated by a reduction in the opioid induced K+ current. Expand
Physiological properties of central medial and central lateral amygdala neurons.
The physiological properties of CE medial and CE lateral amygdala neurons are characterized using whole cell recordings in brain slices maintained in vitro to indicate that the CE nucleus contains several types of neurons endowed with distinct physiological properties. Expand