Subregion- and Cell Type–Restricted Gene Knockout in Mouse Brain

@article{Tsien1996SubregionAC,
  title={Subregion- and Cell Type–Restricted Gene Knockout in Mouse Brain},
  author={J. Tsien and D. Chen and D. Gerber and Cindy Tom and E. Mercer and David J. Anderson and M. Mayford and E. Kandel and S. Tonegawa},
  journal={Cell},
  year={1996},
  volume={87},
  pages={1317-1326}
}
Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact… Expand

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