Submillisecond unfolding kinetics of apomyoglobin and its pH 4 intermediate.

  • M Jamin, S R Yeh, +1 author R L Baldwin
  • Published 1999 in Journal of molecular biology
Submillisecond mixing experiments and tryptophan fluorescence spectroscopy are used to address two questions raised in earlier stopped-flow studies of the folding and unfolding kinetics of sperm whale apomyoglobin. A study of the pH 4 folding intermediate (I) revealed, surprisingly, that its folding and unfolding kinetics are measurable and fit the two… (More)