Corpus ID: 27157897

Submerged Production and Characterization of Grifola frondosa Polysaccharides – A New Application to Cosmeceuticals

  title={Submerged Production and Characterization of Grifola frondosa Polysaccharides – A New Application to Cosmeceuticals},
  author={Sang Woo Kim and Hyeong Jin Hwang and Bum-chun Lee and Jong Won Yun},
  journal={Food Technology and Biotechnology},
Grifola frondosa (maitake) is traditionally called 'the king of mushrooms' and 'the hen of the woods'. Both the fruiting bodies and the mycelium of maitake have been reported to have antitumor and antiviral activities. Recently, submerged culture processes have been developed, with the intention of providing opportunities for increased economic exploitation of maitake. Commonly the aim of these processes is to produce extracellular polysaccharides (EPS), mostly glucans, and to explore their… 
Submerged cultivation of medicinal mushrooms: bioprocesses and products (review).
  • V. Elisashvili
  • Medicine, Biology
    International journal of medicinal mushrooms
  • 2012
An update of the present state of the art and future prospects of submerged cultivation of medicinal mushrooms to produce mycelium and bioactive metabolites is provided to make a contribution for the research and development of new pharmaceutical products from mushrooms.
Basidiomycetes Mushroom Biotechnology for the Development ofFunctional Products: The Effect of Drying Processes on Biological Activity
Ganoderma lucidum, Grifola frondosa and Agaricus blazei mushrooms were cultured in a 14 L bioreactor using low-cost, non-conventional culture media. To produce useful raw material for the development
Bioconversion of Lignocellulosic Agri-Waste into Edible Protein by Mushroom Cultivation and Evaluation of Yield on Different Agri-Residues with Biochemical Composition
Pleurotus ostreatus (Oyster mushroom), a saprophytic macroscopic fungus, can grow on several lignocellulosic substances by degradation activity supported by several enzymes secreted by them. The
Polysaccharides in Grifola frondosa mushroom and their health promoting properties: A review.
The purpose of the present review is to provide systematically reorganized information on structural characteristics, biological activities, and structure-activity relationship of G. frondosa polysaccharides to support their further therapeutic potentials and sanitarian functions.
Optimal conditions for mycelia biomass and extracellular polysaccharides of Grifola frondosa: Effect of agitation speed, inoculum ratio and initial pH
The results show that the culture parameters including agitation speed, inoculum ratio, and initial pH influenced pellet morphology, biomass, and EPS production, which may be useful for future development of fermentation techniques for the production of G. frondosa products.
Recent trends in submerged cultivation of mushrooms and their application as a source of nutraceuticals and food additives
Abstract Nowadays, mushroom bioactive compounds production by submerged cultivation has attracted great interest. Controlling culture conditions in bioreactors makes submerged cultivation more
Optimization of cellulase-assisted extraction process and antioxidant activities of polysaccharides from Tricholoma mongolicum Imai.
Water-soluble polysaccharides extracted from T. mongolicum Imai by cellulase-assisted extraction and their antioxidant activities were investigated, suggesting that TMIPs are potential natural antioxidants for use in functional foods.
Development of high yielding strain of Pleurotus tuber-regium: fructification, nutritional and phylogenetic studies
Proximate analysis of the sclerotium showed that the mutant, Pt30, had improved nutritional compositions compared to the wild strain with a total non-structural carbohydrate concentration of 2.41 g as against 0.93 g, and it was established that mutagenesis is capable of improving P. tuber-regium for a successful commercial venture in sclerotsia production.
Applied modern biotechnology for cultivation of Ganoderma and development of their products
A white-rot basidiomycete Ganoderma spp. has long been used as a medicinal mushroom in Asia, and it has an array of pharmacological properties for immunomodulatory activity. There have been many
Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa.
A water-soluble polysaccharide was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography and its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR and 13C NMR analysis.


Biological activities of the polysaccharides produced from submerged culture of the edible Basidiomycete Grifola frondosa
Five groups of polysaccharides were prepared from mycelium extract and top and bottom fraction of filtrate precipitates by submerged culture of Grifola frondosa at two different media (glucose and
Production and molecular characteristics of four groups of exopolysaccharides from submerged culture of Phellinus gilvus.
The SEC/MALLS analysis revealed that Fr-I, which has extremely high molecular weight, was presumably an aggregate of complex polysaccharides forming a compact globular shape; whereas Fr-II was nearly spherical, Fr-III and Fr-IV were rod-like chains in an aqueous solution.
Characterization of the antitumor glucan obtained from liquid-cultured Grifola frondosa.
Antitumor glucan (grifolan LE) was purified from LELFD by diethylaminoethyl-Sephadex A-25 chromatography and ethanol precipitation and showed antitumor activity against sarcoma 180 cells in ICR mice.
Fractionation and Characterization of Antitumor Polysaccharides from Maitake, Grifola frondosa
Three groups of polysaccharides from the edible mushroom “Maitake,” the cultured fruiting body of Grifola frondosa, were extracted with hot water, 3% NH4-oxalate (100°C), and 5% sodium hydroxide
Production of exopolysaccharide from mycelial culture of Grifola frondosa and its inhibitory effect on matrix metalloproteinase-1 expression in UV-irradiated human dermal fibroblasts.
Results suggest that EPS obtained from mycelial culture of G. frondosa HB0071 may contribute to inhibitory action in photoaging skin by reducing the MMP 1-related matrix degradation system.
Production and characterization of exopolysaccharides from submerged culture of Phellinus linteus KCTC 6190
Abstract The optimal temperature and initial pH for both mycelial growth and exopolysaccharide (EPS) production in shake flask cultures of Phellinus linteus KCTC 6190 were found to be 30 °C and pH
Polysaccharopeptides of Coriolus versicolor: physiological activity, uses, and production.
This review focuses on the physiologically active polysaccharopeptides of C. versicolor, which are effective immunopotentiators which are used to supplement the chemotherapy and radiotherapy of cancers and various infectious diseases.
Submerged culture conditions for the production of mycelial biomass and exopolysaccharides by the edible Basidiomycete Grifola frondosa
Optimization of submerged culture conditions for mycelial biomass and exopolysaccharide (EPS) production by Grifola frondosa was studied and the most desirable morphology for EPS production was a feather-likeMycelial clump.
Immunostimulating Activity of Polysaccharides from Mycelia of Phellinus linteus Grown under Different Culture Conditions
Activity was increased by compared with polysaccharide which was extracted from mycelia grown on medium with glucose, suggesting that activity could be increased by poly Saccharide modification through changes in physiological conditions.
The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from
Flow cytometry analysis on cell cycle and cell death (apoptosis) of Molt 4 cells indicated that the anticancer mechanism of PSP is related to its ability to induce S-phase cell arrest and apoptosis, respectively.