Subgrouping of DR4 alleles by DNA heteroduplex analysis.

  title={Subgrouping of DR4 alleles by DNA heteroduplex analysis.},
  author={Rosanna Sorrentino and Isabella Cascino and Roberto Tosi},
  journal={Human immunology},
  volume={33 1},
Amplified DNA molecules from six DR4 alleles at the DRB1 locus were denatured and cross-hybridized pairwise. Several of the DNA heteroduplexes thus generated were found to possess distinct mobilities in polyacrylamide gel electrophoresis. The degree of retardation as compared to homoduplexes depends strongly on the position of mismatched nucleotide pairs. In critical positions, the type of mispairing also influences the number of heteroduplex bands since a transversion-type substitution yields… CONTINUE READING