Subfractionation of Chlamydomonas 18 S dynein into two unique subunits containing ATPase activity.

Abstract

The 18 S dynein of the outer arm of Chlamydomonas flagella contains two different heavy polypeptide chains (Mr approximately equal to 340,000), two intermediate chains (Mr = 69,000 and 78,000), and eight light chains (Mr = 8,000-20,000). We report here that when purified 18 S dynein is dialyzed against a low ionic strength solution, it is dissociated into… (More)

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