Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material

@article{Lawo2012SubdiffractionIO,
  title={Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material},
  author={S. Lawo and Monica Hasegan and G. Gupta and L. Pelletier},
  journal={Nature Cell Biology},
  year={2012},
  volume={14},
  pages={1148-1158}
}
The centrosome is the main microtubule organization centre of animal cells. It is composed of a centriole pair surrounded by pericentriolar material (PCM). Traditionally described as amorphous, the architecture of the PCM is not known, although its intricate mode of assembly alludes to the presence of a functional, hierarchical structure. Here we used subdiffraction imaging to reveal organizational features of the PCM. Interphase PCM components adopt a concentric toroidal distribution of… Expand
Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization
TLDR
It is demonstrated that the pericentriolar material is organized into two main structural domains: a layer juxtaposed to the centriole wall, and proteins extending farther away from the Centrosome organized in a matrix, using SIM and STORM subdiffraction-resolution microscopies. Expand
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The centrosome is the major microtubule-organizing centre of animal cells. It participates in a number of crucial cellular functions including cell motility, intracellular transport, mitotic spindleExpand
Amorphous no more: subdiffraction view of the pericentriolar material architecture.
TLDR
Current knowledge of PCM architecture and assembly is outlined, emphasizing recent super-resolution imaging studies that revealed the PCM has a layered structure made of fibers and matrices conserved from flies to humans and debunk the long-standing view of an amorphous PCM. Expand
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The centrosome is an unusual organelle that lacks a surrounding membrane, raising the question of what limits its size and shape. Moreover, while electron microscopy (EM) has provided a detailed viewExpand
Pericentriolar material structure and dynamics
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TLDR
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Condensation of pericentrin proteins in human cells illuminates phase separation in centrosome assembly
TLDR
This work has found that endogenously expressed pericentrin (PCNT), a conserved PCM scaffold protein, condenses into dynamic granules during late G2/early mitosis before incorporating into mitotic centrosomes and proposes that CCs and LCRs are preserved under evolutionary pressure in part to mediate liquid-liquid phase separation. Expand
Molecular architecture of a cylindrical self-assembly at human centrosomes
The cell is constructed by higher-order structures and organelles through complex interactions among distinct structural constituents. The centrosome is a membraneless organelle composed of twoExpand
A molecular mechanism of mitotic centrosome assembly in Drosophila
TLDR
This study shows that the centriole protein Asl initiates the recruitment of DSpd-2 and Cnn to mother centrioles; both proteins then assemble into co-dependent scaffold-like structures that spread outwards from the mother centRIole and recruit most, if not all, other PCM components. Expand
Centriole-less pericentriolar material serves as a microtubule organizing center at the base of C. elegans sensory cilia
TLDR
It is shown that SPD-5, the functional homolog of CDK5RAP2, is essential for embryonic mitosis, while SPD-2/CEP192 and PCMD-1, which are essential in the one-cell embryo, are dispensable. Expand
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It is demonstrated that the pericentriolar material is organized into two main structural domains: a layer juxtaposed to the centriole wall, and proteins extending farther away from the Centrosome organized in a matrix, using SIM and STORM subdiffraction-resolution microscopies. Expand
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