Subconductance states in single-channel activity of skeletal muscle ryanodine receptors after removal of FKBP12.

@article{Ahern1997SubconductanceSI,
  title={Subconductance states in single-channel activity of skeletal muscle ryanodine receptors after removal of FKBP12.},
  author={Gerard P. Ahern and Pauline R. Junankar and Angela F Dulhunty},
  journal={Biophysical journal},
  year={1997},
  volume={72 1},
  pages={146-62}
}
FKBP12 was removed from ryanodine receptors (RyRs) by incubation of rabbit skeletal muscle terminal cisternae membranes with rapamycin. The extent of FKBP12 removal was estimated by immunostaining Western blots of terminal cisternae proteins. Single FKBP12-depleted RyR channels, incorporated into planar lipid bilayers, were modulated by Ca2+, ATP, ryanodine, and ruthenium red in the cis chamber and opened frequently to the normal maximum conductance of approximately 230 pS and to substate… CONTINUE READING

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