Subcompartments of the G1 phase of cell cycle detected by flow cytometry.

  title={Subcompartments of the G1 phase of cell cycle detected by flow cytometry.},
  author={Zbigniew Darzynkiewicz and Thomas K. Sharpless and Lisa F Staiano-Coico and Myron R. Melamed},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={77 11},
Cellular DNA and RNA were simultaneously quantitated in individual cells of cycling and quiescent populations by flow cytometry. Based on differences in RNA content, two distinct subcompartments, G1A and G1B, were recognized in the G1 phase of exponentially growing cells. After mitosis, cells reside in the low-RNA, G1A compartment from which they cannot enter the S phase directly. An increase in RNA above a critical level is required for G1 cells to be able to initiate DNA replication; G1 cells… CONTINUE READING