Subcellular transport and ribosomal incorporation of microinjected protein S6 in oocytes from Xenopus laevis.

@article{Kalthoff1982SubcellularTA,
  title={Subcellular transport and ribosomal incorporation of microinjected protein S6 in oocytes from Xenopus laevis.},
  author={Holger Kalthoff and Dietmar M. Richter},
  journal={Biochemistry},
  year={1982},
  volume={21 4},
  pages={741-5}
}
Protein S6 was isolated from 40S ribosomal subunits of Xenopus laevis oocytes, labeled with sodium boro[3H]hydride, and microinjected back into oocytes. In the first 4 h of incubation, the uptake of S6 into the nucleus increased to a maximum, with, however, no detectable incorporation into 40S ribosomal subunits. After this lag period, S6 was progressively integrated into the small ribosomal subunit. When rRNA transcription was inhibited by actinomycin D, the uptake of S6 into the nucleus and… CONTINUE READING