Subcellular transcriptomics-dissection of the mRNA composition in the axonal compartment of sensory neurons.

@article{Minis2014SubcellularTO,
  title={Subcellular transcriptomics-dissection of the mRNA composition in the axonal compartment of sensory neurons.},
  author={Adi Minis and Dvir Dahary and Ohad Manor and Dena Leshkowitz and Yitzhak Pilpel and Avraham Yaron},
  journal={Developmental neurobiology},
  year={2014},
  volume={74 3},
  pages={365-81}
}
RNA localization is a regulatory mechanism that is conserved from bacteria to mammals. Yet, little is known about the mechanism and the logic that govern the distribution of RNA transcripts within the cell. Here, we present a novel organ culture system, which enables the isolation of RNA specifically from NGF dependent re-growing peripheral axons of mouse embryo, sensory neurons. In combination with massive parallel sequencing technology, we determine the subcellular localization of most… CONTINUE READING
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