Subcellular positioning of small molecules.

@article{Takayama2001SubcellularPO,
  title={Subcellular positioning of small molecules.},
  author={Shuichi Takayama and Emanuele Ostuni and Philip Leduc and Keiji Naruse and Donald E. Ingber and George M Whitesides},
  journal={Nature},
  year={2001},
  volume={411 6841},
  pages={1016}
}
L ocalized perturbation of processes that take place inside the living cell depends on molecular and spatial discrimination on a micrometre scale. Here we report the use of multiple laminar streams in a microfluidic channel to deliver membrane-permeable molecules to selected subcellular microdomains. This technique opens up avenues for non-invasively visualizing, probing and manipulating the cellular metabolic and structural machinery. We have applied this method (called 'partial treatment of… CONTINUE READING
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