Subcellular organization of calcium signalling in hepatocytes and the intact liver.


Hepatocytes respond to inositol 1,4,5-trisphosphate (InsP3)-linked agonists with frequency-modulated oscillations in the intracellular free calcium concentration ([Ca2+]i), that occur as waves propagating from a specific origin within each cell. The subcellular distribution and functional organization of InsP3-sensitive Ca2+ pools has been investigated, in both intact and permeabilized cells, by fluorescence imaging of dyes which can be used to monitor luminal Ca2+ content and InsP3-activated ion permeability in a spatially resolved manner. The Ca2+ stores behave as a luminally continuous system distributed throughout the cytoplasm. The structure of the stores, an important determinant of their function, is controlled by the cytoskeleton and can be modulated in a guanine nucleotide-dependent manner. The nuclear matrix is devoid of Ca2+ stores, but Ca2+ waves in the intact cell propagate through this compartment. The organization of [Ca2+]i signals has also been investigated in the perfused liver. Frequency-modulated [Ca2+]i oscillations are still observed at the single cell level, with similar properties to those in the isolated hepatocyte. The [Ca2+]i oscillations propagate between cells in the intact liver, leading to the synchronization of [Ca2+]i signals across part or all of each hepatic lobule.


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@article{Thomas1995SubcellularOO, title={Subcellular organization of calcium signalling in hepatocytes and the intact liver.}, author={Anu P. Thomas and Dominique C Renard-Rooney and Gy{\"{o}rgy Hajn{\'o}czky and Lawrence D. Robb-Gaspers and Chi-Shen Lin and Tim Rooney}, journal={Ciba Foundation symposium}, year={1995}, volume={188}, pages={18-35; discussion 35-49} }