Subcellular localization of renal kallikrein by ultrastructural immunocytochemistry.

  title={Subcellular localization of renal kallikrein by ultrastructural immunocytochemistry.},
  author={Carlos P Vio and Carlos D. Figueroa},
  journal={Kidney international},
  volume={28 1},
The subcellular distribution of immunoreactive kallikrein was described in the rat nephron using ultrastructural immunocytochemistry. The renal tissue was fixed with a mixture of buffered picric acid-paraformaldehyde-glutaraldehyde and immunostained with the peroxidase-antiperoxidase method for the electron microscope with the following steps: antikallikrein antiserum, anti-IgG serum, peroxidase-antiperoxidase complex, 3-3' diaminobenzidine-H2O2, and post-staining with osmium tetroxide… CONTINUE READING