Subcellular localization of oxidosqualene cyclases from Arabidopsis thaliana, Trypanosoma cruzi, and Pneumocystis carinii expressed in yeast

@article{Milla2002SubcellularLO,
  title={Subcellular localization of oxidosqualene cyclases from Arabidopsis thaliana, Trypanosoma cruzi, and Pneumocystis carinii expressed in yeast},
  author={Paola Milla and Franca Viola and Simonetta Oliaro Bosso and Flavio Rocco and Luigi Cattel and Bridget M. Joubert and Ren{\'e}e J Leclair and Seiichi P T Matsuda and Gianni Balliano},
  journal={Lipids},
  year={2002},
  volume={37},
  pages={1171-1176}
}
Cycloartenol synthase from Arabidopsis thaliana and lanosterol synthase from Trypanosoma cruzi and Pneumocystis carinii were expressed in yeast, and their subcellular distribution in the expressing cells was compared. Determination of enzymatic (oxidosqualene cyclase, OSC) activity and SDS-PAGE analysis of subcellular fractions proved that enzymes from T. cruzi and A. thaliana have high affinity for lipid particles, a subcellular compartment rich in triacylglycerols, and steryl esters… 
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38 Citations

Inhibitory Effect of Umbelliferone Aminoalkyl Derivatives on Oxidosqualene Cyclases from S. cerevisiae, T. cruzi, P. carinii, H. sapiens, and A. thaliana: a Structure–Activity Study
TLDR
Eighteen coumarin derivatives were tested as inhibitors of oxidosqualene cyclases from Saccharomyces cerevisiae, Trypanosoma cruzi, Pneumocystis carinii, Homo sapiens, and Arabidopsis thaliana and suggest the present series as a promising compound family for the development of novel antiparasitic agents.
Functional Characterization and Localization of Pneumocystis carinii Lanosterol Synthase
TLDR
Using fluorescence microscopy, it is shown for the first time the presence of apparent lipid particles in P. carinii and the localization of PcErg7p to cholesterol particles in yeast, providing strong evidence that the enzyme serves a similar function in the P.Carinii sterol pathway.
Analogs of squalene and oxidosqualene inhibit oxidosqualene cyclase of Trypanosoma cruzi expressed in Saccharomyces cerevisiae
TLDR
The expression in yeast cells could be a safe and easy model for studying the activity and the selectivity of the potential inhibitors of T. cruzi OSC, and the expression of proteins of pathogenic organisms in yeast seems very promising for preliminary screening of compounds that have potential therapeutic activity.
Umbelliferone aminoalkyl derivatives as inhibitors of oxidosqualene cyclases from Saccharomyces cerevisiae, Tripanosoma cruzi, and Pneumocystis carinii
TLDR
Results identify a particularly promising new family of OSC inhibitors, for the development of novel antiparasitic agents, including derivatives bearing a methylallylaminoalkyl flexible chain.
Difference in the late ergosterol biosynthesis between yeast spheroplasts and intact cells.
TLDR
A comparative study on post-squalene sterol synthesis in intact yeast cells and spheroplasts was carried out with strains from three genera as well as with engineered S. cerevisiae cells altered in regard to the late ergosterol synthesis pathway to search for an answer.
Pneumocystis carinii Sterol 14α‐Demethylase Activity in Saccharomyces cerevisiae erg11 Knockout Mutant: Sterol Biochemistry
TLDR
Detailed sterol analysis of wild‐type Saccharomyces cerevisiae and in an erg11 knockout mutant expressing either P. carinii or S. Cerevisiae 14DM indicated that the mutant transformed with erg11 showed more 14DM activity than wild‐ type yeast and suggested that the yeast in the yeast cell utilizes 4α‐methyl sterols better than the P.carinii enzyme.
Plant Oxidosqualene Metabolism: Cycloartenol Synthase–Dependent Sterol Biosynthesis in Nicotiana benthamiana
TLDR
A CAS1-specific functional sterol pathway in engineered yeast, and a strict dependence on CAS1 of tobacco sterol biosynthesis are shown.
Sterol biosynthesis and sterol uptake in the fungal pathogen Pneumocystis carinii.
Members of the fungal genus Pneumocystis colonize healthy mammalian hosts without causing apparent disease, but colonization in immunocompromised hosts may result in a potentially fatal pneumonia…
Identification of marneral synthase, which is critical for growth and development in Arabidopsis.
  • Y. Go, S. Lee, M. Suh
  • Environmental Science
    The Plant journal : for cell and molecular biology
  • 2012
TLDR
Evidence is provided that the unusual triterpenoid pathway via marneral synthase is important for the growth and development of Arabidopsis.
Enfumafungin synthase represents a novel lineage of fungal triterpene cyclases
TLDR
The preliminary identification of the enfumafungin biosynthetic gene cluster (BGC) is reported based on genome sequencing, phylogenetic reconstruction, gene disruption, and cDNA sequencing studies, suggesting that efuA represents the first member of a widespread lineage of fungal SHCs.
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References

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TLDR
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TLDR
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TLDR
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TLDR
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