Subcellular localization of glycogen synthase with monoclonal antibodies.

  title={Subcellular localization of glycogen synthase with monoclonal antibodies.},
  author={Richard D. Lane and M G Hegazy and E. M. Reimann},
  journal={Biochemistry international},
  volume={18 5},
Two monoclonal antibodies, designated 7H5 and 8E11, were produced against glycogen synthase purified from rabbit skeletal muscle. Both antibodies were of the IgG1 (k) isotype. Western blot analysis of extracts of rat and rabbit tissues showed that antibody 7H5 recognized glycogen synthase from skeletal and cardiac muscles, but not from liver. Antibody 8E11 gave similar results but the responses were weaker. Antibody 7H5 also recognized a 69,000 dalton tryptic fragment of glycogen synthase… CONTINUE READING