Subcellular localization of benzodiazepine/GABAA receptors in the cerebellum of rat, cat, and monkey using monoclonal antibodies.

@article{Somogyi1989SubcellularLO,
  title={Subcellular localization of benzodiazepine/GABAA receptors in the cerebellum of rat, cat, and monkey using monoclonal antibodies.},
  author={Peter Somogyi and Hiroshi Takagi and J. Grayson Richards and Hanns Mohler},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={1989},
  volume={9 6},
  pages={2197-209}
}
Two monoclonal antibodies, bd-17 and bd-24, specific for the beta- and alpha-subunit of the GABAA/benzodiazepine receptor/chloride channel complex, respectively, were used to determine the subcellular distribution of immunoreactivity in the cerebellum by electron microscopy. The 2 antibodies showed similar antigen distribution on the plasma membrane (except in the rat; bd-24 does not recognize the rat antigen), but intracellular immunoreactivity was more prevalent for the alpha-subunit. The… CONTINUE READING

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Granule cells showed the strongest immunoreactivity , not only on their dendrites which receive synapses from GABA - containing Golgi cell terminals , but also on their somata which do not receive synapses .
Granule cells showed the strongest immunoreactivity , not only on their dendrites which receive synapses from GABA - containing Golgi cell terminals , but also on their somata which do not receive synapses .
Granule cells showed the strongest immunoreactivity , not only on their dendrites which receive synapses from GABA - containing Golgi cell terminals , but also on their somata which do not receive synapses .
Granule cells showed the strongest immunoreactivity , not only on their dendrites which receive synapses from GABA - containing Golgi cell terminals , but also on their somata which do not receive synapses .
Nerve EndingsSibling in is aNeuroglia
No immunoreactivity was detected on axons , nerve terminals , or glial cells .
NeurogliaSibling in is aNerve Endings
No immunoreactivity was detected on axons , nerve terminals , or glial cells .
The results demonstrate that different neuronal types express the GABAA / benzodiazepine receptor / chloride channel complex to different degrees .
Two monoclonal antibodies , bd-17 and bd-24 , specific for the beta- and alpha - subunit of the GABAA / benzodiazepine receptor / chloride channel complex , respectively , were used to determine the subcellular distribution of immunoreactivity in the cerebellum by electron microscopy .
The results demonstrate that different neuronal types express the GABAA / benzodiazepine receptor / chloride channel complex to different degrees .
Two monoclonal antibodies , bd-17 and bd-24 , specific for the beta- and alpha - subunit of the GABAA / benzodiazepine receptor / chloride channel complex , respectively , were used to determine the subcellular distribution of immunoreactivity in the cerebellum by electron microscopy .
The results demonstrate that different neuronal types express the GABAA / benzodiazepine receptor / chloride channel complex to different degrees .
Two monoclonal antibodies , bd-17 and bd-24 , specific for the beta- and alpha - subunit of the GABAA / benzodiazepine receptor / chloride channel complex , respectively , were used to determine the subcellular distribution of immunoreactivity in the cerebellum by electron microscopy .
The results demonstrate that different neuronal types express the GABAA / benzodiazepine receptor / chloride channel complex to different degrees .
Two monoclonal antibodies , bd-17 and bd-24 , specific for the beta- and alpha - subunit of the GABAA / benzodiazepine receptor / chloride channel complex , respectively , were used to determine the subcellular distribution of immunoreactivity in the cerebellum by electron microscopy .
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