Subcellular localization of 14-3-3 proteins in Toxoplasma gondii tachyzoites and evidence for a lipid raft-associated form.

@article{Assossou2003SubcellularLO,
  title={Subcellular localization of 14-3-3 proteins in Toxoplasma gondii tachyzoites and evidence for a lipid raft-associated form.},
  author={Olga Assossou and Françoise Besson and J Rouault and Florence Persat and Christine Brisson and Laurent Duret and Josette Ferrandiz and Martine Mayençon and François Peyron and St{\'e}phane Picot},
  journal={FEMS microbiology letters},
  year={2003},
  volume={224 2},
  pages={
          161-8
        }
}
A polyclonal antibody was raised against a Toxoplasma gondii 14-3-3-gluthatione S-transferase fusion protein obtained by cloning a 14-3-3 cDNA sequence determined from the T. gondii database. This antibody specifically recognized T. gondii 14-3-3 without any cross-reaction with mammalian proteins. Immunofluorescence microscopy studies of the tachyzoites or the T. gondii-infected cells suggested cytosolic and membranous localizations of 14-3-3 protein. Different subcellular fractions were… CONTINUE READING

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