Subcellular localization and functional expression of the glycerol uptake protein 1 (GUP1) of Saccharomyces cerevisiae tagged with green fluorescent protein.

Abstract

GFP (green fluorescent protein) from Aequorea victoria was used as an in vivo reporter protein when fused to the N- and C-termini of the glycerol uptake protein 1 (Gup1p) of Saccharomyces cerevisiae. The subcellular localization and functional expression of biologically active Gup1-GFP chimaeras was monitored by confocal laser scanning and electron… (More)

Topics

Cite this paper

@article{Bleve2005SubcellularLA, title={Subcellular localization and functional expression of the glycerol uptake protein 1 (GUP1) of Saccharomyces cerevisiae tagged with green fluorescent protein.}, author={Gianluca Bleve and Giuseppe Zacheo and Maria Stella Cappello and Franco Dellaglio and Francesco Grieco}, journal={The Biochemical journal}, year={2005}, volume={390 Pt 1}, pages={145-55} }