Subcellular compartmentation of dolichol taken up by mouse leukemia cells.


[1-3H]Dolichol was intraperitoneally incubated with mouse leukemia Lcb 35 cells. The radioactive lipid taken up by the cells was subsequently recovered in two fractions separated by sucrose density gradient centrifugation: a particular, most probably, lysosomal fraction and a cytoplasmic one. Thin-layer chromatography of radioactive lipids present in both… (More)


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