Subcellular Metabolite and Lipid Analysis of Xenopus laevis Eggs by LAESI Mass Spectrometry

@inproceedings{Shrestha2014SubcellularMA,
  title={Subcellular Metabolite and Lipid Analysis of Xenopus laevis Eggs by LAESI Mass Spectrometry},
  author={Bindesh Shrestha and Prabhakar Sripadi and Brent R. Reschke and Holly Henderson and Matthew J. Powell and Sally A Moody and Akos Vertes},
  booktitle={PloS one},
  year={2014}
}
Xenopus laevis eggs are used as a biological model system for studying fertilization and early embryonic development in vertebrates. Most methods used for their molecular analysis require elaborate sample preparation including separate protocols for the water soluble and lipid components. In this study, laser ablation electrospray ionization (LAESI), an ambient ionization technique, was used for direct mass spectrometric analysis of X. laevis eggs and early stage embryos up to five cleavage… CONTINUE READING