Subcellular Distribution of Actively Partitioning F Plasmid during the Cell Division Cycle in E. coli

@article{Niki1997SubcellularDO,
  title={Subcellular Distribution of Actively Partitioning F Plasmid during the Cell Division Cycle in E. coli},
  author={Hironori Niki and Sota Hiraga},
  journal={Cell},
  year={1997},
  volume={90},
  pages={951-957}
}
F plasmid is partitioned with fidelity to daughter cells during cell division cycle owing to two trans-acting genes, sopA and sopB, and a cis-acting site, sopC. We visualized the subcellular distribution of mini-F-plasmid molecules by fluorescence in situ hybridization. Mini-F-plasmid molecules having the sopABC segment were localized at midcell in newborn cells. Replicated plasmid molecules migrated to cell positions 1/4 and 3/4 without coupling with cell elongation and were tethered to these… CONTINUE READING
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