Sub-cellular immunolocalization of the glucosinolate sinigrin in seedlings of Brassica juncea

@article{Kelly1998SubcellularIO,
  title={Sub-cellular immunolocalization of the glucosinolate sinigrin in seedlings of Brassica juncea},
  author={Patrick D. Kelly and Atle M. Bones and John Trevor Rossiter},
  journal={Planta},
  year={1998},
  volume={206},
  pages={370-377}
}
Polyclonal rat antibodies were raised to a bovine serum albumin-sinigrin conjugate and used to immunolocalize sinigrin (2-propenylglucosinolate) in imbibed seeds and developing seedlings of Brassica juncea. (L.) Czern. Sinigrin was localized to protein bodies in aleurone-like cells but shown to be absent from myrosin cells. Double labelling techniques were used to co-localize both myrosinase (β-thioglucoside glucohydrolase, EC 3.2.3.1) and sinigrin. Myrosin grains were labelled only with the… CONTINUE READING
39 Citations
20 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 39 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 20 references

The myrosinaseglucosinolate system , its organisation and biochemistry

  • AM Bones, JT Rossiter
  • Physiol Plant
  • 1996

Puri ® cation and characterization of a nitrilase from Brassica napus

  • LA Bestwick, LM Gronning, DC James, A Bones, JT Rossiter
  • Physiol Plant
  • 1993

Temporal , cellspeci ® c , and tissuepreferential expression of myrosinase genes during embryo and seedling development in Sinapis alba

  • SH Yiu, FW Collins, I FulcherRGAltosaar
  • Planta
  • 1993

Myrosinase is localized to the interior of myrosin grains and is not associated to the surrounding tonoplast membrane

  • AS Hùglund, M Lenman, K Rask
  • Plant Sci
  • 1992

Development and characteristics of myrosinase in Brassica napus during early seedling growth

  • DC James, JT Rossiter
  • Physiol Plant
  • 1991

Immuno - gold localization of indole3acetic acid in peach seedlings

  • M Sharma
  • Plant Cell Physiol
  • 1990

Immunocytochemical localization of myrosinase in Brassica napus L

  • EW Underhill
  • Planta
  • 1990

Immunolog - ical characterisation of rapeseed myrosinase

  • M Lenman, M Rodin, Josefsson L-G, L Rask
  • Eur J Biochem
  • 1990

Enzyme - linked immunosorbent assays for alkenyl glucosinolates

  • F Hassan, NE Rothnie, SP Yeung, MV Palmer
  • J Agric Food Chem
  • 1988

Similar Papers

Loading similar papers…