Style self-incompatibility gene products of Nicotlana alata are ribonucleases

@article{McClure1989StyleSG,
  title={Style self-incompatibility gene products of Nicotlana alata are ribonucleases},
  author={Bruce McClure and Volker R Haring and Paul R Ebert and Marilyn A. Anderson and Richard J. Simpson and Fumio Sakiyama and Adrienne Elizabeth Clarke},
  journal={Nature},
  year={1989},
  volume={342},
  pages={955-957}
}
SELF-INCOMPATIBILITY in flowering plants is often controlled by a single nuclear gene (the S-gene) having several alleles1. This gene prevents fertilization by self-pollen or by pollen bearing either of the two S- alleles expressed in the style. Sequence analysis shows that three alleles of the S gene of Nicotiana alata encode style glycoproteins2,3 with regions of defined homology. Two of the homologous regions also show precise homology with ribonucleases T2 (ref. 4) and Rh (ref. 5). We… Expand
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Pollen-expressed S-RNases are not involved in self-incompatibility in Lycopersicon peruvianum
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Self-incompatibility in solanaceous plants is gametophytically controlled by a multiallelic S-locus and it is demonstrated that the S3-RNase is not the pollen product of the S 3-allele. Expand
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TLDR
It is concluded that S2-gene expression correlates well with the expression of incompatibility, and is reported here that expression of the gene occurs in the stigma and throughout the secretory tissue, but not in other parts, of mature pistils. Expand
Sequence variability of three alleles of the self-incompatibility gene of Nicotiana alata.
Three alleles of the self-incompatibility gene of Nicotiana alata have been cloned and sequenced. A comparison of the sequences shows a surprisingly low level of homology (56%) and the presence ofExpand
Identification, isolation, and N-terminal sequencing of style glycoproteins associated with self-incompatibility in Nicotiana alata.
TLDR
S-Gene-associated glycoproteins from styles of Nicotiana alata were purified by cation exchange fast protein liquid chromatography with yields of 0.5 to 8 micrograms of protein per style, depending on the S-genotype of the plant, and showed high degree of homology with previously published sequences. Expand
Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata
TLDR
A complementary DNA clone encoding a style protein of Nicotiana alata which segregates with the S2 allele has now been sequenced and shows that the S-allele-associated style components in different genotypes of N. alata and in Lycopersicon peruvianum are homologous. Expand
Inhibition of in Vitro Pollen Tube Growth by Isolated S-Glycoproteins of Nicotiana alata.
TLDR
Pollen from three S-genotypes of Nicotiana alata was grown in vitro in the presence of S-glycoproteins isolated from styles of the same three genotypes, but pollen tube growth of all genotypes was inhibited. Expand
Barley aleurone layers secrete a nuclease in response to gibberellic Acid : purification and partial characterization of the associated ribonuclease, deoxyribonuclease, and 3'-nucleotidase activities.
TLDR
It is concluded that in response to gibberellic acid, barley aleurone tissue secretes a nuclease having ribonuclease, deoxyribonucleasing, and 3'-nucleotidase activities. Expand
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TLDR
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TLDR
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