Stuffer-free multiplex ligation-dependent probe amplification based on conformation-sensitive capillary electrophoresis: a novel technology for robust multiplex determination of copy number variation.

@article{Shin2012StufferfreeML,
  title={Stuffer-free multiplex ligation-dependent probe amplification based on conformation-sensitive capillary electrophoresis: a novel technology for robust multiplex determination of copy number variation.},
  author={Gi Won Shin and Seung-Hyun Jung and Seon-Hee Yim and Boram Chung and Gyoo Yeol Jung and Yeun-Jun Chung},
  journal={Electrophoresis},
  year={2012},
  volume={33 19-20},
  pages={3052-61}
}
Developing diagnostic tools based on the application of known disease/phenotype-associated copy number variations (CNVs) requires the capacity to measure CNVs in a multiplex format with sufficient reliability and methodological simplicity. In this study, we developed a reliable and user-friendly multiplex CNV detection method, termed stuffer-free MLPA-CE-SSCP, that combines a variation of multiplex ligation-dependent probe amplification (MLPA) with CE-SSCP. In this variation, MLPA probes were… CONTINUE READING
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