Studying vertebrate topoisomerase 2 function using a conditional knockdown system in DT40 cells

@inproceedings{Johnson2009StudyingVT,
  title={Studying vertebrate topoisomerase 2 function using a conditional knockdown system in DT40 cells},
  author={Mark Johnson and Hui Hui Phua and Sophia C. Bennett and Jennifer M. Spence and Christine J. Farr},
  booktitle={Nucleic acids research},
  year={2009}
}
DT40 is a B-cell lymphoma-derived avian cell line widely used to study cell autonomous gene function because of the high rates with which DNA constructs are homologously recombined into its genome. Here, we demonstrate that the power of the DT40 system can be extended yet further through the use of RNA interference as an alternative to gene targeting. We have generated and characterized stable DT40 transfectants in which both topo 2 genes have been in situ tagged using gene targeting, and from… CONTINUE READING
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