Studying the intracellular dissociation of polymer-oligonucleotide complexes by dual color fluorescence fluctuation spectroscopy and confocal imaging.

@article{Lucas2005StudyingTI,
  title={Studying the intracellular dissociation of polymer-oligonucleotide complexes by dual color fluorescence fluctuation spectroscopy and confocal imaging.},
  author={Bart Lucas and Katrien Remaut and Niek N Sanders and Kevin Braeckmans and Stefaan C. De Smedt and Joseph Demeester},
  journal={Biochemistry},
  year={2005},
  volume={44 29},
  pages={9905-12}
}
To transfect cells, cationic polymers as well as cationic liposomes are widely investigated as carriers for both oligonucleotides and plasmid DNA. A major step in the successful intracellular delivery of the DNA is the release from its carrier. In this study, dual color fluorescence fluctuation spectroscopy (dual color FFS) was explored in order to characterize the intracellular dissociation of cationic polymer/oligonucleotide complexes. As a model, rhodamine green-labeled oligonucleotides… CONTINUE READING