Studying the effect of single mismatches in primer and probe binding regions on amplification curves and quantification in real-time PCR.

@article{Suess2009StudyingTE,
  title={Studying the effect of single mismatches in primer and probe binding regions on amplification curves and quantification in real-time PCR.},
  author={Beate Suess and Gabriele Flekna and Martin Wagner and Ingeborg Hein},
  journal={Journal of microbiological methods},
  year={2009},
  volume={76 3},
  pages={316-9}
}
Comparison of a broad range of characteristics of real-time PCR amplification curves yielded only slight alterations for low numbers of mismatches in the primer binding regions, resulting in a quantification error up to 63.12%. The effects were more pronounced for mismatches in the probe binding region and resulted in a quantification error up to 33%. 

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References

Publications referenced by this paper.
Showing 1-10 of 24 references

Sequence variation can affect the performance of minor groove binder TaqMan probes in viral diagnostic assays.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology • 2006
View 4 Excerpts
Highly Influenced

Sequence variation in primer targets affects the accuracy of viral quantitative PCR.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology • 2005
View 4 Excerpts
Highly Influenced

Mfold web server for nucleic acid folding and hybridization prediction

Nucleic Acids Research • 2003
View 2 Excerpts
Highly Influenced

Gene expression profiles in gallbladder cancer: the close genetic similarity seen for early and advanced gallbladder cancers may explain the poor prognosis

J. H. Kim, H. N. Kim, +5 authors A. W. Lowe
Tumour Biol • 2008
View 2 Excerpts

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