Studying enzyme-beta-lactam interactions using X-ray diffraction.
@article{Kelly1989StudyingEI, title={Studying enzyme-beta-lactam interactions using X-ray diffraction.}, author={Judith A. Kelly and James R. Knox and H. Zhao}, journal={Journal of molecular graphics}, year={1989}, volume={7 2}, pages={ 87-92 } }
The interaction of representative beta-lactam antibiotics with a bacterial enzyme target has been mapped in three dimensions using X-ray diffraction data to 2.25 A resolution. Examination of complexes of cephalosporin C, benzylmonobactam, and alpha-(2,3)-methylenepenicillin G with the D-alanyl-D-alanine transpeptidase-carboxypeptidase from Streptomyces R61 shows that the enzyme's reactive serine has acylated the beta-lactam ring of each inhibitor. The known half-lives of the three acyl…