Study on the purification of growth hormone-like substance from pituitaries of the snake Ptyas mucosa.

@article{Ng1993StudyOT,
  title={Study on the purification of growth hormone-like substance from pituitaries of the snake Ptyas mucosa.},
  author={Tzi Bun Ng and H M Lee and C. H. K. Cheng and C. C. Wong},
  journal={Preparative biochemistry},
  year={1993},
  volume={23 3},
  pages={
          351-61
        }
}
Extract from the snake (Ptyas mucosa) pituitaries was capable of inhibiting the binding of 125I-labelled bovine growth hormone to female rat liver membranes. The growth hormone-like substance was not adsorbed on Concanavalin A-Sepharose nor DEAE-cellulose, but could be purified by gel filtration on Sephadex G-50. It possessed a molecular weight of about 19,000 as judged by SDS-polyacrylamide gel electrophoresis. The iodinated snake growth hormone-like substance bound to membranes prepared from… Expand
1 Citations
Hormones and Reproductive Cycles in Snakes
TLDR
This chapter attempts to provide a broad overview of the effects of hormones on reproduction in snakes, focusing on seminal studies, and Suggestions for future research are provided. Expand

References

SHOWING 1-10 OF 11 REFERENCES
Partial purification of prolactin-like substance from snake (Ptyas mucosa) pituitaries.
Pituitary extract of the common rat snake (Ptyas mucosa) was found to be capable of displacing the binding of 125I-labelled ovine prolactin to female rat liver membranes, suggesting the presence ofExpand
Purification and properties of teleost growth hormone.
TLDR
Results from these investigations demonstrated a significant degree of cross-reaction between Tilapia GH and pituitary extract from another teleost (perch), but purified tetrapod GHs were essentially nonreactive. Expand
Purification and radioimmunoassay of chicken growth hormone.
TLDR
A specific homologous radioimmunoassay has been developed using the avian growth hormone, which had a similar isoelectric point, molecular weight and amino acid composition to mammalian growth hormone. Expand
Purification and properties of reptilian and amphibian growth hormones.
TLDR
Characterization studies with high purified growth hormone isolated from the pituitaries of two reptilian species and two amphibian species indicate that many elements of growth hormone structure have been strongly conserved during evolution. Expand
Presence of prolactin receptors in kidney and large intestine of the snake Ptyas mucosa.
TLDR
The present study is the first report of the presence of prolactin receptors in snake kidney and large intestine membranes. Expand
Isolation of prolactin from salmon pituitary.
TLDR
The results suggest that the higher molecular weight proteins are aggregates formed during isolation but the 23,000 molecular weight protein may be a modified form of the unadsorbed prolactin. Expand
Binding of human growth hormone to rat liver membranes: Lactogenic and somatotropic sites
TLDR
In membranes of male rats the somatotropic sites account for virtually all the binding sites for hGH, whereas in those of female animals, they represent only a small fraction of the hGH binding sites. Expand
Identification and characterization of high and low affinity binding sites for growth hormone in rabbit liver.
TLDR
The specific binding of rat (r) and rabbit (rb) [l25I]iodo-GH was studied in liver membrane preparations isolated from male, virgin female, late pregnant, and midlactating rabbits and suggested the existence of at least two classes of binding sites for both GHs. Expand
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four majorExpand
Rat hepatic prolactin receptor: pubertal exposure to sex steroids alters responsivity to testosterone.
TLDR
Exposure of the ovariectomized rats during puberty to mestranol at the dose used did not restore the normal adult female rat's level of hepatic prolactin receptor, but these rats did become more resistant to subsequent testosterone challenge in adulthood. Expand
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