Study on the purification of growth hormone-like substance from pituitaries of the snake Ptyas mucosa.

  title={Study on the purification of growth hormone-like substance from pituitaries of the snake Ptyas mucosa.},
  author={Tzi Bun Ng and H M Lee and C. H. K. Cheng and C. C. Wong},
  journal={Preparative biochemistry},
  volume={23 3},
Extract from the snake (Ptyas mucosa) pituitaries was capable of inhibiting the binding of 125I-labelled bovine growth hormone to female rat liver membranes. The growth hormone-like substance was not adsorbed on Concanavalin A-Sepharose nor DEAE-cellulose, but could be purified by gel filtration on Sephadex G-50. It possessed a molecular weight of about 19,000 as judged by SDS-polyacrylamide gel electrophoresis. The iodinated snake growth hormone-like substance bound to membranes prepared from… Expand
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