Study on the photo-generation of superoxide radicals in Photosystem II with EPR spin trapping techniques

@article{Zhang2004StudyOT,
  title={Study on the photo-generation of superoxide radicals in Photosystem II with EPR spin trapping techniques},
  author={Suping Zhang and Junjie Weng and Jingxi Pan and Tiecheng Tu and Side Yao and Chun-he Xu},
  journal={Photosynthesis Research},
  year={2004},
  volume={75},
  pages={41-48}
}
Direct EPR evidence of the photo-generation of superoxide radicals (O2−.) was obtained by using a novel spin trapping probe in spinach Photosystem II (PS II) membrane fragments. The production of O2−. was detected by following the formation of 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO) superoxide adducts (DEPMPO-OOH). The inhibition of O2−. formation by 3-(3,4-dichlorophenyl) -1,1-dimethylurea (DCMU) and the 77 K fluorescence spectrum indicated that O2−. were generated from PS… Expand
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  • P. Pospíšil
  • Medicine, Chemistry
  • Biochimica et biophysica acta
  • 2009
TLDR
The review points out the mechanistic aspects in the production of ROS on both the electron acceptor and electron donor side of PSII. Expand
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