Corpus ID: 19392249

Study on the lipid organization of stratum corneum lipid models by (cryo-) electron diffraction.

@article{Pilgram1998StudyOT,
  title={Study on the lipid organization of stratum corneum lipid models by (cryo-) electron diffraction.},
  author={Gonneke S. K. Pilgram and A M Engelsma-van Pelt and Gert T Oostergetel and Henk K. Koerten and Joke A Bouwstra},
  journal={Journal of lipid research},
  year={1998},
  volume={39 8},
  pages={
          1669-76
        }
}
The barrier function of the skin resides in the stratum corneum (SC). This outermost layer consists of protein-rich corneocytes and lipid-rich intercellular domains. These domains form the rate-limiting step for transepidermal water loss and the penetration of substances from the environment. To study the nature of the barrier function, stratum corneum lipid models have been examined with wide-angle X-ray diffraction. A disadvantage of this technique is that it requires bulk quantities of lipid… Expand
Electron diffraction provides new information on human stratum corneum lipid organization studied in relation to depth and temperature.
TLDR
It is concluded that the lipids in human stratum corneum are highly ordered throughout the stratum Corneum and that electron diffraction allows monitoring of the local lipid organization, which contributes to the understanding of stratumCorneum barrier function. Expand
Fatty acid interdigitation in stratum corneum model membranes: a neutron diffraction study
The influence of the chain length of the free fatty acid (FFA) in a stratum corneum (SC) lipid model membrane composed of N-(α-hydroxyoctadecanoyl)-phytosphingosine (CER [AP]), cholesterol (Ch), FFAExpand
Study of human stratum corneum and extracted lipids by thermomicroscopy and DSC.
A study on the thermal behavior of human stratum corneum and lipids is described. The use of high scanning rate DSC for both SC and extracted lipids allows the consistent determination of transitionExpand
Structure of the skin barrier and its modulation by vesicular formulations.
TLDR
In order to unravel the mechanisms involved in increasing the drug transport across the skin, information on the effect of vesicles on drug permeation rate, the permeation pathway and perturbations of the skin ultrastructure is of importance. Expand
Oleic acid disorders stratum corneum lipids in Langmuir monolayers.
TLDR
Results indicate that lower concentrations of OA preferentially mix with and disorder the ceramide-enriched domains, followed by perturbation of the PA-en enriched domains and disruption of SC lipid domain separation at higher OA levels. Expand
Molecular dynamics simulations of stratum corneum lipid models: fatty acids and cholesterol.
TLDR
Analysis of the simulations indicates that the free fatty acid fraction of stratum corneum lipids stays in a highly ordered crystalline state at skin temperatures, which is strongly influenced when cholesterol is added. Expand
Ceramides and Skin Function
TLDR
The link between skin disorders and changes in barrier lipid composition, especially in ceramides, is difficult to prove, however, most skin disorders that have a diminished barrier function present a decrease in total ceramide content with some differences in the ceramide pattern. Expand
Thermal phase transition behavior of lipid layers on a single human corneocyte cell.
TLDR
The improved electron diffraction method used to analyze the dynamic structural change in a single corneocyte cell non-invasively stripped off from human skin surface suggests that the structural features of the lipid layers are well preserved after the mechanical stripping of the corneocytes. Expand
The influence of two azones and sebaceous lipids on the lateral organization of lipids isolated from human stratum corneum.
The main problem with topical application of compounds to administer drugs to and regulate drug levels in a human body, is the barrier formed by the intercellular lipid matrix of the stratum corneumExpand
The role of ceramide composition in the lipid organisation of the skin barrier.
TLDR
The results of the present study clearly indicate that at an equimolar CHOL:CER molar ratio the lamellar organisation is least sensitive to a variation in CER composition, while at a reduced CHol:Cer molar ratios the Cer composition plays a more prominent role in the lameLLar phases. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 40 REFERENCES
Cryo‐electron diffraction as a tool to study local variations in the lipid organization of human stratum corneum
TLDR
Improved the microscope settings and developed a new preparation method to study ex vivo human SC by cryo‐electron diffraction based on the conventional tape‐stripping method and offers the possibility to study depth‐related changes in the lipid organization of human SC. Expand
Structure of lamellar lipid domains and corneocyte envelopes of murine stratum corneum. An X-ray diffraction study.
TLDR
By X-ray diffraction, the structure of the intercellular domains and the extracted lipids of murine stratum corneum and the protein envelopes of the corneocytes are surveyed, and patterns suggest a beta-pleated sheet organizational scheme. Expand
Lipid organization in pig stratum corneum.
TLDR
The results led us to conclude that the intercellular lipids in the stratum corneum are organized in at least two different lamellar structures with repeat distances of 6 and 13.2 nm. Expand
X-ray diffraction analysis of isolated skin lipids: reconstitution of intercellular lipid domains.
TLDR
Results indicate that a structure with dimensions similar to those of the lamellar repeating unit found in skin stratum corneum does not depend on the presence of protein but does depend onThe presence of specific skin ceramides and appropriate concentrations of cholesterol and fatty acid. Expand
Visualization and electron diffraction on cryosections of stratum corneum: a utopia?
The skin acts as an effective barrier to protect the body against penetration of substances from the environment and against desiccation. The main barrier function resides in the stratum corneum, andExpand
Oriented structure in human stratum corneum revealed by X-ray diffraction.
TLDR
The studies confirm the presence of a still-unknown protein component existing in the beta form that would be located either inside the corneocytes or in some dilatated zones of the intercellular spaces. Expand
Human stratum corneum lipids have a distorted orthorhombic packing at the surface of cohesive failure.
  • Y. Chen, T. Wiedmann
  • Materials Science, Medicine
  • The Journal of investigative dermatology
  • 1996
TLDR
Although proteins are present, the fracture plane of the stratum corneum is largely composed of lipids that appear to have a distorted orthorhombic packing. Expand
Phase behavior of stratum corneum lipids in mixed Langmuir-Blodgett monolayers.
TLDR
The results of this investigation suggest that the model system used in this study is in good agreement with those of other studies concerning the phase behavior of the stratum corneum lipids. Expand
Structural investigations of human stratum corneum by small-angle X-ray scattering.
TLDR
The structure of human stratum corneum was investigated with small-angle X-ray scattering (SAXS) and it is concluded that in the original curve the lipids are arranged in two unit cells with repeat distances of 6.4 nm and 13. Expand
Membrane structures in normal and essential fatty acid-deficient stratum corneum: characterization by ruthenium tetroxide staining and x-ray diffraction.
TLDR
The new RuO4 protocol provides quantitative information about SC lamellar dimensions and morphologic abnormalities in bilayer distribution and substructure in EFAD stratum corneum that are not detected by either x-ray diffraction or computer-aided image reconstruction. Expand
...
1
2
3
4
...