Study on reproductive endocrinology of human placenta--culture of highly purified cytotrophoblast cell in serum-free hormone supplemented medium.

Abstract

A new method of long-term culture of cytotrophoblast cells in serum-free medium has been developed. Cytotrophoblast cells were isolated with cold trypsin and purified by unit gravity sedimentation through BSA density gradients. The cells were cultured in the FD medium with supplement of EGF, insulin, transferrin and sodium selenite. They could survive over… (More)

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