Study on relationship between expression level and molecular conformations of gene drugs targeting to hepatoma cells in vitro.

Abstract

AIM To increase exogenous gene expression level by modulating molecular conformations of targeting gene drugs. METHODS The full length cDNAs of both P(40) and P(35) subunits of human interleukin 12 were amplified through polymerase chain reaction (PCR) and cloned into eukaryotic expressing vectors pcDNA3.1(+/-) to construct plasmids of P(+)/IL-12, P(+)/P… (More)

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