Study on heterogeneity of beta-glucosidase from Aspergillus species by using counter-current distribution.

Abstract

beta-Glucosidase plays a key role in cellulose degradation by cleaving its cellobiose units to glucose. The heterogeneity of the enzyme has been studied earlier by isoelectric focusing and several isoforms were found in the culture filtrate of Trichoderma and Aspergillus species. An aqueous two-phase system is an alternative fractionation method in which… (More)

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