• Chemistry
  • Published 2010

Study on expression of Hax-1 human keratinocyte line HaCaT by Hax-1 short hairpin RNA

@inproceedings{Hua2010StudyOE,
  title={Study on expression of Hax-1 human keratinocyte line HaCaT by Hax-1 short hairpin RNA},
  author={Zhong Zhi Hua},
  year={2010}
}
Objective To study the function of HS1-associated protein X-1(Hax-1)shRNA in inducing apoptosis of human keratinocyte line HaCaT.Methods ShRNA expression vector pSilencer-Hax-1 was constructed.Reverse transcription-polymerase chain reaction(RT-PCR) technique and Western blot were used to analyze mRNA and protein expressions of Hax-1 in transfected HaCaT cells.And then apoptotic HaCaT cells were detected by flow cytometry.Results Expression of the mRNA and protein of Hax-1 was down-regulated and… CONTINUE READING