• Chemistry
  • Published 2009

Study on Rapidly Simultaneous Detection of Deoxynivalenol and Zearalenone

@inproceedings{Jue2009StudyOR,
  title={Study on Rapidly Simultaneous Detection of Deoxynivalenol and Zearalenone},
  author={Zhang Jue and Gao Lei and Zhou Bin and Zhang Yi and Huang Biao and Jin Jian},
  year={2009}
}
Objective:A high sensitive indirect competitive dual-label time-resolved fluoroimmunoassay (TRFIA) was established to simultaneously detect deoxynivalenol and Zearalenone in cereal. Methods:DON-BSA and ZEN-BSA were co-coated in 96-well plates as the solid phase antigen to compete with free DON and ZEN for limited anti-DON polyclonal antibody or anti-ZEN monoclonal antibody. The antibodies of goat anti-rabbit and goat anti-mouse were labeled with the Eu3+-and Sm3+-chelates respectively,used as… CONTINUE READING