• Biology
  • Published 2013

Study of the phylogenetic evolution of five members of Anopheles maculatus s.l.with the mtDNA-COI gene fragment

@inproceedings{Linhua2013StudyOT,
  title={Study of the phylogenetic evolution of five members of Anopheles maculatus s.l.with the mtDNA-COI gene fragment},
  author={Tang Lin-hua},
  year={2013}
}
Objectives To sequence and compare the mtDNA-COⅠ variance of the 5 members of Anopheles maculatus s.l.(An.pseudowillmori,An.maculatus s.s,An.willmori,An.dravidicus,and An.sawadwongporni) and analyze their phylogenetic relationship.Methods An.maculates s.l.was identified by morphology and multiple PCR with variance in the ITS2 gene sequence,and then mtDNA-COⅠ was amplified and sequenced.Chromas and Bioedit software were used to compare the gene sequence and Mega 3.0 was used to construct a… CONTINUE READING