Study of the human keratinocyte isolation methods and in vitro culture techniques in a single laboratory

  title={Study of the human keratinocyte isolation methods and in vitro culture techniques in a single laboratory},
  author={Esther Garcia Fernandez and Jacoob Bejar and A. I. Alonso-Varona and M. D. Garc{\'i}a Masdevall and F. J. Gabilondo},
  journal={European Journal of Plastic Surgery},
The survey was conducted to analyze different human keratinocyte isolation methods and in vitro culture techniques used in current practice. The aim was to find which would work best in our laboratory to obtain epidermal sheets suitable for grafting. The techniques for epidermal removal and keratinocyte isolation involve dispase II or trypsin solutions at different concentrations and times. The number of cells and cellular viability were measured from different origins and size samples. The… CONTINUE READING

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